LAL Reagents: Lyophilized amebocyte lysate (LAL) is an aqueous extract of blood cells (amebocytes) from the Atlantic horseshoe crab.
TAL reagent: TAL reagent is an aqueous extract of blood cells from Tachypleus tridentatus.
At present, the main production of TAL reagents are in the United States, Japan, China and other countries.
Gel clot endotoxin test assay: ( Generally Bioendo gel clot single test and gel clot multi test )
When operation the test for confirmation of labeled lysate sensitivity, the result is abnormal, 2 Lamada not gel formation?
Check whether the preparation method of the bacterial control standard endotoxin is correct. Vortex mixing is required for each diluent (see the instruction manual or called insert of the control standard endotoxin in details.) and check whether the lysate reagent matches the control standard endotoxin.
It is recommended to use a water bath or a dry heat incubator for the constant temperature process of the lysate reagent (for example, an incubator or a dry box is not effective). During the incubation, water environment in the water bath that is static and stable, all vibrations are avoided. Pay special attention to the water bath to turn off the water flow pump.
Judging the result, gently take out the test tube or glass ampoule from the thermostat, slowly invert 180 degrees, the gel formation in the tube will not be deformed, and will not slip off, the result was recorded by signal ”+”; no gel formation is formed or the gel clot can not be kept intact even though it is formed. The wall slip is negative, the result was recorded by signal ”-”.
How to operate the endotoxin test assay to test the sample?
First of all, the test for confirmation of labeled lysate sensitivity should be carried out. To confirm the sensitivity is the same to the label marks.
For sample analysis, conduct pre-interference endotoxin test assays.
To operate the complete endotoxin assay of “ interference test ”.
To operate the limit test for the getting how is the endotoxins level in the sample.
Rapid gel clot endotoxin test Kit:
Inthe rapid gel clot endotoxin testing, the sample positive control does not generate gel?First, use bacterial endotoxin test water to verify the lysate reagents, ensure the operators and the environment have meet the regulations;
If the kit is qualified, then the positive control of the sample does not gel formation due to the inhibitory effect of the sample, and the sample should be processed and be treated. The most common sample processing method is dilution.